Vergleich des Regenerativen Zytokinprofils von Adipose Derived Stromal Cells (ASCs) Gewonnen Mittels Abdominoplastik, Suction Assisted Liposuction (SAL) und Ultrasound Assisted Liposuction (UAL)

Zusammenfassung

Einleitung Mesenchymale Stromazellen sind aufgrund ihres regenerativen Potentials schon in zahlreichen Fachgebieten der Medizin im klinischen Einsatz. In der Plastischen Chirurgie stehen vor allem Fettstammzellen (Adipose derived stromal cells = ASCs) aus Lipoasopirat im Mittelpunkt der rezenten Forschung. Die Fähigkeit zur Ausschüttung von regenerativen Zytokinen ist der Schlüsselmechanismus im therapeutischen Einsatz von ASCs. Es sind zurzeit eine Vielzahl an Methoden und Geräten zur Lipoaspiratgewinnung in der klinischen Anwendung. Bei den meisten dieser Geräte sind die Auswirkungen auf die regenerativen Eigenschaften der ASCs noch weitgehend ungeklärt. Ziel dieser Studie ist der Vergleich der Expression von wesentlichen regenerativen Zytokinen von ASCs, gewonnen mittels dreier Standardverfahren der Plastischen Chirurgie: Abdominoplastik, Suction assisted Liposuction (SAL) und Ultrasound assisted Liposuction (UAL).

Patienten und Methoden UAL und SAL Lipoaspirate wurden jeweils von 3 gesunden weiblichen Probanden gewonnen, be vor eine Abdominoplastik an denselben Patienten durchgeführt wurde. ASCs wurden aus UAL und SAL Lipoaspiraten sowie aus den Abdominoplastikresektaten isoliert und die RNA-Expression von wichtigen regenerativen Zytokinen (HGF (hepatocyte growth factor), FGF-2 (basic fibroblast growth factor), MCP-1 (monocyte chemotactic protein 1), SDF-1 (stromal cell-derived factor 1) und VEGF (vascular endothelial growth factor) verglichen.

Resultate Es zeigte sich kein Unterschied hinsichtlich der Expression von HGF, FGF-2, SDF-1 und VEGF zwischen den einzelnen Proben, jedoch eine signifikant erhöhte Expression von MCP-1 in ASCs gewonnen aus UAL-Lipoaspirat.

Conclusio UAL stellt bezüglich Einfluss auf das ASC Expressionsprofil von Schlüsselzytokinen der Gewebserneuerung eine mit SAL zumindest vergleichbare Methode dar. Sowohl UAL als auch SAL Lipoaspirate sind geeignet zur Gewinnung von funktionellen ASCs vergleichbar mit minimal manipulierten Zellen aus Abdominoplastikresektaten.

Abstract

Introduction Mesenchymal stem cells are already used in numerous areas of medicine. In the field of plastic surgery, the main focus of recent research has been to utilise adipose-derived stromal cells (ASCs), mainly harvested via liposuction. The ability to release regenerative cytokines is supposed to be the key mechanism for the therapeutic efficacy of ASCs. There are currently a variety of methods and devices in clinical use for harvesting lipoaspirate and consequently ASCs. For most of these devices, the effect on the regenerative cytokine profile of ASCs is unknown. The aim of this study is to compare the expression of key regenerative cytokines of ASCs derived by three standard procedures of plastic surgery: abdominoplasty, suction-assisted liposuction (SAL) and ultrasound-assisted liposuction (UAL).

Patients and methods UAL and SAL lipoaspirates were obtained from 3 healthy female volunteers before abdominoplasty was performed in the same patients. ASCs were isolated from UAL and SAL lipoaspirates as well as from abdominoplasty resections and RNA expression of important regenerative cytokines (HGF (hepatocyte growth factor), FGF-2 (basic fibroblast growth factor), MCP-1 (monocyte chemotactic protein 1), SDF-1 (stromal cell-derived factor 1) and VEGF (vascular endothelial growth factor) was compared.

Results There was no difference in the expression of HGF, FGF-2, SDF-1 and VEGF between the individual samples, but we were able to demonstrate significantly increased expression of MCP-1 in ASCs obtained from UAL lipoaspirate.

Conclusion UAL and SAL lipoaspirates are suitable for obtaining functional ASCs and are comparable to minimally manipulated cells from abdominoplasty resections.

Schlüsselwörter

Adipose derived stem cells (ASCs) - Liposuction - Zytokine - Abdominoplastik - Suction Assisted Liposuction (SAL) - Ultrasound Assisted Liposuction (UAL)

Key words

adipose derived stem cells (ASCs) - liposuction - cytokine - ultrasound assisted liposuction - suction assisted liposuction - abdominoplasty

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